Investigation of Apoptotic Biomarkers in Dental Pulp Stem Cells under Hypoxic Condition using RNA-Seq

Philip William Cannizzaro

Immature teeth can have halted root development through pathology associated with caries, trauma, or dental anomalies such as dens evaginatus. Pulp tissue under stress induces ischemia and subsequent necrosis. Through regenerative endodontic procedures, proper procedural protocol often leads to revitalization following blood clot formation. The aim of this study is focused on understanding the roles of caspase proteins within the inflammasome reaction leading to pulp necrosis by evaluating expression profile levels through RNA sequencing technology. Human dental pulp tissue was extracted from healthy adult third molars extracted prophylactically and healthy adult premolars extracted for orthodontics collected with IRB exemption. Tissue samples purified for mRNA allows expression analysis evaluation. DESeq2 and Limma analysis promoted statistical analysis of expression profile to determine whether or not significant differences in transcription and likely subsequent translation between diseased and healthy tissue. The study identified 27 genes above six-fold higher expression under hypoxia conditions, these markers include the following: TUBA1B, ACTB, NFKB, CTSD, CTSB, MCL1, JUN, TUBA1C, and others.